The study which spanned 35 days was carried out to investigate the impact of using probiotics on broiler birds' blood protein profile using the gel protein band development comparison. CRD was used for the experiment. A total of 36 Cobb Vantress birds were raised and fed with probiotics brand (poultry growth enhancer) containing culture solution of Lactobacillus spp. i.e milk bacteria, Bacillus spp., and Saccharomyces cerevisiae. The bird physical parameter such as body weight and feed supplied were weighed and birds were fed with restricted feeding style. The bird fed with probiotics included diet had a significantly higher average daily weight gain than those not fed with probiotics (p<0.05) at 40.16 for the treated and 39.09 for the control while the final weight gain on the restricted diet with treatment was not significantly different at 1.13 while those without the treatment was 1.167 and the initial body weight of the control birds was higher than those fed with probiotics in the diet. The electrophoretic protein profile of control and probiotics-fed birds based on a preliminary investigation of 10 randomly selected birds fed with and without probiotics. It was discovered that thicker bands were observed at point for globulins, transferrins, and albumins showing increased level as globulins contain antibiotics for defense, transferrins for oxygen transport, and albumins for greater blood oncotic pressure; this suggests that probiotics had a major impact on the birds' health
growth performance, blood serum, protein profile, broiler finisher, probiotics
Abd El‐Hack, M. E., El‐Saadony, M. T., Shafi, M. E., Qattan, S. Y., Batiha, G. E., Khafaga, A. F., ... & Alagawany, M. (2020). Probiotics in poultry feed: A comprehensive review. Journal of animal physiology and animal nutrition, 104(6), 1835-1850.
Abdel-Hafeez, H. M., Saleh, E. S., Tawfeek, S. S., Youssef, I. M., & Abdel-Daim, A. S. (2017). Effects of probiotic, prebiotic, and synbiotic with and without feed restriction on performance, hematological indices and carcass characteristics of broiler chickens. Asian-Australasian journal of animal sciences, 30(5), 672.
Adhikari, P., Lee, C. H., Cosby, D. E., Cox, N. A., & Kim, W. K. (2019). Effect of probiotics on fecal excretion, colonization in internal organs and immune gene expression in the ileum of laying hens challenged with SalmonellaEnteritidis. Poultry science, 98(3), 1235-1242.
Agyare, C., Boamah, V. E., Zumbi, C. N., & Osei, F. B. (2018). Antibiotic use in poultry production and its effects on bacterial resistance. Antimicrobial resistance—A global threat, 33-51.
Alagawany, M., El-Hack, A., Mohamed, E., Farag, M. R., Sachan, S., Karthik, K., & Dhama, K. (2018). The use of probiotics as eco-friendly alternatives for antibiotics in poultry nutrition. Environmental Science and Pollution Research, 25(11), 10611-10618.
Alayande, K. A., Aiyegoro, O. A., & Ateba, C. N. (2020). Probiotics in animal husbandry: Applicability and associated risk factors. Sustainability, 12(3), 1087.
Arsène, M. M., Davares, A. K., Andreevna, S. L., Vladimirovich, E. A., Carime, B. Z., Marouf, R., & Khelifi, I. (2021). The use of probiotics in animal feeding for safe production and as potential alternatives to antibiotics. Veterinary World, 14(2), 319.
Barutta, F., Bellini, S., Kimura, S., Hase, K., Corbetta, B., Corbelli, A., ... & Gruden, G. (2022). Protective effect of the tunneling nanotube-TNFAIP2/M-sec system on podocyte autophagy in diabetic nephropathy. Autophagy, 1-20.
Bruinsma, J. (2003). World agriculture: towards 2015/2030: an FAO perspective. Earthscan. Food and Agriculture Organization, London/Rome.
Dowarah, R., Verma, A. K., & Agarwal, N. (2017). The use of Lactobacillus as an alternative of antibiotic growth promoters in pigs: A review. Animal Nutrition, 3(1), 1-6.
Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics, 11(1), 1-42.
Ezema, C. (2013). Probiotics in animal production: A review. Journal of Veterinary Medicine and Animal Health, 5(11), 308-316.
Ferreira, J. T., Grant, W. S., & Avtalion, R. R. (1984). Workshop on Fish Genetics. CSIR Special Publications, Pretoria.
Fijan, S. (2014). Microorganisms with claimed probiotic properties: an overview of recent literature. International journal of environmental research and public health, 11(5), 4745-4767.
Hammed, A. M., Fashina-Bombata, H. A., & Omitogun, G. O. (2011). Biochemical Characterization of an ecotype cichlid from Epe Lagoon ‘ Wesafu’ and Oreochromis niloticus using Sodium dodecyl sulphate polyacrylamide gel electrophoresis. Research in Biotechnology, 2(5).
Heak, C., Sukon, P., & Sornplang, P. (2018). Effect of direct-fed microbials on culturable gut microbiotas in broiler chickens: a meta-analysis of controlled trials. Asian-Australasian journal of animal sciences, 31(11), 1781.
Ikhimiukor, O. O., Odih, E. E., Donado-Godoy, P., & Okeke, I. N. (2022). A bottom-up view of antimicrobial resistance transmission in developing countries. Nature Microbiology, 1-9.
İncili, G. K., Karatepe, P., Akgöl, M., Güngören, A., Koluman, A., İlhak, O. İ., ... & Hayaloğlu, A. A. (2022). Characterization of lactic acid bacteria postbiotics, evaluation in-vitro antibacterial effect, microbial and chemical quality on chicken drumsticks. Food Microbiology, 104, 104001.
Keeley, B., Little, C., & Zuehlke, E. (2019). The State of the World's Children 2019: Children, Food and Nutrition--Growing Well in a Changing World. UNICEF.
Kühn, S., Milasi, S., & Yoon, S. (2018). Population ageing and future labour market challenges. World Employment and Social Outlook, 2018(1), 45-50.
Lan, P. T. N., Sakamoto, M., & Benno, Y. (2004). Effects of two probiotic Lactobacillus strains on jejunal and cecal microbiota of broiler chicken under acute heat stress condition as revealed by molecular analysis of 16S rRNA genes. Microbiology and immunology, 48(12), 917-929.
Lillehoj, H., Liu, Y., Calsamiglia, S., Fernandez-Miyakawa, M. E., Chi, F., Cravens, R. L., ... & Gay, C. G. (2018). Phytochemicals as antibiotic alternatives to promote growth and enhance host health. Veterinary research, 49(1), 1-18.
Manyi-Loh, C., Mamphweli, S., Meyer, E., & Okoh, A. (2018). Antibiotic use in agriculture and its consequential resistance in environmental sources: potential public health implications. Molecules, 23(4), 795.
Markowiak, P. (2017). Śliżewska К. Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients, 9(9), 1021-1051.
Molina-Flores, B., Manzano-Baena, P., & Coulibaly, M. D. (2020). The role of livestock in food security, poverty reduction and wealth creation in West Africa. FAO.
Moorby, J. M., & Fraser, M. D. (2021). New feeds and new feeding systems in intensive and semi-intensive forage-fed ruminant livestock systems. Animal, 15, 100297.
Okutucu, B., Dınçer, A., Habib, Ö., & Zıhnıoglu, F. (2007). Comparison of five methods for determination of total plasma protein concentration. Journal of biochemical and biophysical methods, 70(5), 709-711.
Plaza-Diaz, J., Ruiz-Ojeda, F. J., Gil-Campos, M., & Gil, A. Mechanisms of action of probiotics. Adv Nutr 2019; 10 (suppl_1): S49-66. External Resources Crossref (DOI).
Popp, J., Lakner, Z., Harangi-Rakos, M., & Fari, M. (2014). The effect of bioenergy expansion: Food, energy, and environment. Renewable and sustainable energy reviews, 32, 559-578.
Ramos-Vivas, J., Tapia, O., Elexpuru-Zabaleta, M., Pifarre, K. T., Armas Diaz, Y., Battino, M., & Giampieri, F. (2022). The Molecular Weaponry Produced by the Bacterium Hafnia alvei in Foods. Molecules, 27(17), 5585.
Ricke, S. C. (2021). Prebiotics and alternative poultry production. Poultry Science, 100(7), 101174.
Salim, H. M., Kang, H. K., Akter, N., Kim, D. W., Kim, J. H., Kim, M. J., ... & Kim, W. K. (2013). Supplementation of direct-fed microbials as an alternative to antibiotic on growth performance, immune response, cecal microbial population, and ileal morphology of broiler chickens. Poultry science, 92(8), 2084-2090.
Sender, R., Fuchs, S., & Milo, R. (2016). Revised estimates for the number of human and bacteria cells in the body. PLoS biology, 14(8), e1002533.
Shabani, R., Nosrati, M., Javandel, F., & Kioumarsi, H. (2012). The effect of probiotics on carcass and internal organs of broilers. Annals of Biological Research, 3(12), 5475-5477.
Shivani, K., Sidharath, D., & Rajesh, R. (2017). Effect of probiotic supplementation in broiler birds offered feed formulated with lower protein densities. International Journal of Livestock Research, 7(2), 1-13.
Steel, R. G., & Torrie, J. H. (1985). Bioestadística: principios y procedimientos. McGraw-Hill.
Swann, P. Are Probiotics Doing More Harm Than Good?.
Tang, S. G. H., Sieo, C. C., Ramasamy, K., Saad, W. Z., Wong, H. K., & Ho, Y. W. (2017). Performance, biochemical and haematological responses, and relative organ weights of laying hens fed diets supplemented with prebiotic, probiotic and synbiotic. BMC veterinary research, 13(1), 1-12.Bajagai, Y. S., Klieve, A. V., Dart, P. J., & Bryden, W. L. (2016). Probiotics in animal nutrition: production, impact and regulation. FAO.
Thantsha, M. S., Mamvura, C. I., & Booyens, J. (2012). Probiotics–what they are, their benefits and challenges. New Advances in the Basic and Clinical Gastroenterology, 21.
Thavasu, P. W., Longhurst, S., Joel, S. P., Slevin, M. L., & Balkwill, F. R. (1992). Measuring cytokine levels in blood. Importance of anticoagulants, processing, and storage conditions. Journal of immunological methods, 153(1-2), 115-124.
Van, T. T. H., Yidana, Z., Smooker, P. M., & Coloe, P. J. (2020). Antibiotic use in food animals worldwide, with a focus on Africa: Pluses and minuses. Journal of global antimicrobial resistance, 20, 170-177.
Wang, J., Ishfaq, M., Guo, Y., Chen, C., & Li, J. (2020). Assessment of probiotic properties of Lactobacillus salivarius isolated from chickens as feed additives. Frontiers in veterinary science, 7, 415.
Xu, D., Fu, L., Pan, D., Chu, Y., Feng, M., Lu, Y., ... & Sun, G. (2022). Role of probiotics/synbiotic supplementation in glycemic control: A critical umbrella review of meta-analyses of randomized controlled trials. Critical reviews in food science and nutrition, 1-19.