Background: In the present investigation rhamnolipid production was attempted with Pseudomonas sp. NBP08 using groundnut oil cake as a substrate under solid state fermentation conditions.
Methods: Optimized conditions for rhamnolipid production using Box-Behnken 43 experimental design were 8.24, 8.49, 7.30, and 31.96 for groundnut cake, inoculation load, pH and temperature, respectively.
Results: Kinetic parameters observed in optimized media are specific growth rate k (h-1) 0.091, time to reach RL max (h-1) 84, biomass X (mg g-1) 8.2, RL max concentration mg g-1 2.2, RL content (%) 11.3 and productivity (mg g-1h-1) 022. Enhanced oil recovery (EOR) experiments showed the highest percentage of hydrocarbon removal of 85.6% was observed at 2% rhamnolipid concentration.
Conclusion: The optimized biosurfactant used in the present study has shown promising results in terms of 85 % hydrocarbon removal, and this can be used for the large-scale bioremediation of hydrocarbon contaminated soils.
Pseudomonas sp. NBP08, rhamnolipid, groundnut oil cake, solid state fermentation, Box-Behnken design, enhanced oil recovery
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